Bilateral Sleeve Fracture of the Patella in a Healthy 11-Year-Old Male: A Case Report.

Bilateral sleeve fracture of the patella (SFP) in skeletally immature children is a rare injury. We report the case of a healthy 11-year-old male who suffered bilateral SFP while playing tag. The avulsed fragments of his left patella were highly comminuted. Open reduction and internal fixation (ORIF) were performed using suture anchors, and the knees were immobilized using a cylinder cast for three weeks. At the one-year follow-up assessment, both knees were found to have regained full strength with no extension lag. However, we observed malunion due to lateral shift of the avulsed fragment, cystic lesions, and clicking in the patella, and the patient experienced residual pain in the left knee. Based on this, we conclude that the sleeve fracture of the patella with comminuted cartilaginous fragments was difficult to treat and might have led to poor clinical results if anatomical reduction and fixation had not been performed.

Prevalence of Cervical Canal Stenosis in Patients with Femoral Fracture: A Retrospective Single-Center Study.

Introduction: Cervical spine surgery reduces falls and subsequent femoral fractures. Nonetheless, current evidence on the prevalence of cervical cord compression (CCC) and increased signal intensity (ISI) in patients with femoral fractures is limited. We aimed to determine the prevalence of CCC and ISI and characterize the physical status and imaging findings using cervical spine magnetic resonance imaging (MRI) and brain computed tomography (CT) in patients with femoral fractures. Methods: This study included 173 patients (140 women, 33 men) with femoral fractures caused by falling, who underwent both cervical spine MRI and brain CT. CCC cases classified as grade 2 (compression of less than one-third of the spinal cord) or higher were investigated. The ISI of the severely affected intervertebral disc level was evaluated using T2-weighted MRI. Hand grip strength and myelopathic signs were also evaluated. Data analysis was performed using the χ2 test, Fisher's exact test, and Student's t-test. Results: Among the 173 patients, 83 (48.0%) had CCC, 29 (16.8%) had ISI, and 68 (39.3%) had abnormal brain CT findings. There was no ISI in patients in the non-CCC group. The patients' average age in the CCC group was significantly higher than that in the non-CCC group. There was no significant difference in the proportion of myelopathic sign and abnormal brain CT findings between the CCC and non-CCC groups or between the ISI and non-ISI groups. Bilateral hand grip strength was significantly negatively correlated with the stenosis rate (right, p=0.047; left, p=0.0018). Conclusions: In conclusion, our study showed that patients with femoral fractures had a high frequency of cervical canal stenosis and intracranial lesions using cervical spine MRI and brain CT.

Treatment Efficacy of Single Topical NSAID (S-Flurbiprofen Plaster) for Knee Symptoms and Locomotive Dysfunction in Knee Osteoarthritis Patients.

OBJECTIVE: The aim of this study was to demonstrate the non-inferiority of S-flurbiprofen plaster (SFPP) monotherapy for treating knee osteoarthritis compared with the combination of conventional oral and topical non-steroidal anti-inflammatory drugs (NSAIDs). METHODS: A total of 222 participants (114, SFPP group; 108, control group) were treated for 4 weeks. The primary endpoint was the change in the degree of pain felt while rising from a chair after 2 and 4 weeks of treatment as determined using the visual analog scale (VAS) . The secondary endpoint was the change in functional scores and test results. Safety was evaluated in terms of the adverse effects. RESULTS: The VAS score significantly decreased in both groups after 2 and 4 weeks of treatment. Non-inferiority in the VAS score was established only at 2 weeks. There were no significant differences in the secondary endpoints between the groups. Skin disorders were more frequent in the SFPP group; however, there was no difference in gastrointestinal (GI) adverse effects. CONCLUSIONS: The therapeutic efficacy of SFPP monotherapy for knee OA, with respect to changes in the VAS, was not shown to be non-inferior to conventional treatment at 4 weeks; however, non-inferiority was established at 2 weeks. The functional improvement in the SFPP group was comparable to that of the control group. No severe GI adverse effects associated with SFPP administration were observed; however, it is necessary to pay more attention to the occurrence of skin disorders with SFPP than with conventional topical NSAIDs.

Anatomical relationship between insertion sites, tunnel placement, and lateral meniscus anterior horn injury during single and double bundle anterior cruciate ligament reconstructions: A comparative macroscopic and histopathological evaluation in cadavers.

PURPOSE: The influence of tunnel extension outside the anatomical anterior cruciate ligament (ACL) insertion in single-bundle (SB) or double-bundle (DB) ACL reconstruction is unclear. This study aimed to investigate the anatomical relationship between ACL insertion and tunnel extension in SB and DB ACL reconstruction, and the impact of tibial tunnel extension to the insertion of anterior horn of lateral meniscus in terms of injury. METHODS: Forty-six paired cadaver knees (mean age, 82.7 ± 10.7 years) were used. Right and left knees were used for SB (10 mm) and DB tunnel reaming (6 mm for the anteromedial and posterolateral bundles). Tibial and femoral tunnels were created to aim at the center of the ACL insertion by arthroscopic visualization. The relationship between tunnel extension and ACL insertion was evaluated macroscopically, and there ratio in two groups were compared by chi-square test. Further, the relative risk for meniscus injury based on tunnel placement was estimated. Coronal section of tibia and parallel section to Blumensaat line in femur were prepared to evaluate the relationship among tunnel position, ACL insertion, and anterior horn of the meniscus histologically. RESULTS: Tibial tunnel extension out of the ACL insertion was observed macroscopically in 9 (39.1%) knees of the SB group, and 3 (13.0%) of the DB group (p = 0.045). In femoral tunnels, extension out of the ACL insertion was seen in 8 (34.8%) knees of the SB group and 1 (4.3%) of the DB group (p = 0.011). Partial injuries of the lateral meniscus anterior horn (LMAH) were observed in 5 (21.7%) knees of the SB group and 1 (4.3%) knee of the DB group (p = 0.091). The relative risk for LMAH injury was calculated as 5.0 (odds ratio, 6.1). Microscopically, SB tunnels appeared to expand out of ACL insertion, both in the femur and tibia. CONCLUSIONS: The incidence of tunnel extension out of the ACL insertion in femur and tibia were higher with SB than with DB reconstruction. Furthermore, injury rate of the LMAH in the DB group was lower.

Biomechanical analysis of medial patellofemoral ligament reconstruction: FiberTape® with knotless anchors versus a semitendinosus tendon autograft with soft anchors.

BACKGROUND: With the use of synthetic materials for medial patellofemoral ligament (MPFL) reconstruction, graft harvest is not necessary and this may facilitate post-operative rehabilitation. The purpose of this study was to compare the structural properties of MPFL reconstruction using a modern synthetic material (FiberTape® (FT), Arthrex) with knotless anchors or a semitendinosus (ST) tendon autograft with soft anchors. METHODS: Nine human fresh-frozen amputated knees were used in this study. After the tensile strength of the native MPFL was measured, the MPFLs were reconstructed using two different surgical procedures, FT with knotless anchors (group A) and a ST with soft anchors (group B). Mechanical testing to failure of the reconstructed MPFLs was performed, and the ultimate load (N), stiffness (N/mm), and failure mode were recorded. RESULTS: The mean (±standard deviation) ultimate load of the native MPFL was 130.6 ± 28.7 N, and all native MPFLs failed at the femoral insertion site. Ultimate load of group A was significantly higher than that of the native MPFL (175.9 ± 34.1 N, p < 0.05). In contrast, the ultimate load of group B was significantly lower than that of the native MPFL (102.7 ± 21.4 N, p < 0.05). The mean stiffness was significantly higher for MPFLs in group A (17.4 ± 4.3 N/mm) than in group B (8.5 ± 1.8 N/mm, p < 0.05). In group A, 5 specimens failed via a knotless anchor pullout at the femoral side, 3 via pullout of knotless anchors at the patella side and 1 via fracture (cheese cut) of the femur without breakage of knotless anchor. In group B, all specimens failed via soft anchor pullout at the patella side. There was no incidence of rupture of FT or ST. CONCLUSION: FT with knotless anchors was stronger than a ST with soft tissue anchors for MPFL reconstruction.

Does Remnant Preservation Influence Tibial Tunnel Enlargement or Graft-to-Bone Integration After Double-Bundle Anterior Cruciate Ligament Reconstruction Using Hamstring Autografts and Suspensory Fixation? A Computed Tomography and Magnetic Resonance Imaging Evaluation.

BACKGROUND: Remnant-preserving anterior cruciate ligament (ACL) reconstruction was introduced to improve clinical outcomes and biological healing. However, the influences of remnant preservation on tibial tunnel position and enlargement are still uncertain. PURPOSE: To evaluate whether remnant-preserving ACL reconstruction influences tibial tunnel position or enlargement and to examine the relationship between tunnel enlargement and graft-to-bone integration in the tibial tunnel. STUDY DESIGN: Cohort study; Level of evidence, 2. METHODS: A total of 91 knees with double-bundle ACL reconstructions were enrolled in this study. ACL reconstruction was performed without a remnant (<25% of the intra-articular portion of the graft) in 44 knees (nonremnant [NR] group) and with remnant preservation in the remaining 47 knees (remnant-preserving [RP] group). Tibial tunnel position and enlargement were assessed using computed tomography (CT). Comparisons between groups were performed. Furthermore, graft-to-bone integration in the tibial tunnel was evaluated using magnetic resonance imaging, and the relationship between tunnel enlargement and graft-to-bone integration at 1 year after ACL reconstruction was assessed. RESULTS: A total of 48 knees (25 in NR group, 23 in RP group) were included; 19 and 24 knees in the NR and RP groups were excluded, respectively, because of graft reruptures and a lack of CT scans. There were no significant between-group differences in tibial tunnel position (P > .05). The degree of posterolateral tunnel enlargement in the axial plane was significantly higher in the RP group than that in the NR group (P = .007) 1 year after ACL reconstruction. The degree of anteromedial tunnel enlargement on axial CT was significantly smaller in knees with graft-to-bone integration than in those without integration (P = .002) 1 year after ACL reconstruction. CONCLUSION: ACL reconstruction with remnant preservation did not influence tibial tunnel position and did not decrease the degree or incidence of tibial tunnel enlargement. At 1 year postoperatively, tunnel enlargement did not affect graft-to-bone integration in the posterolateral tunnel, but graft-to-bone integration was delayed in the anteromedial tunnel.

Greater Laxity in the Anterior Cruciate Ligament-Injured Knee Carries a Higher Risk of Postreconstruction Pivot Shift: Intraoperative Measurements With a Navigation System.

BACKGROUND: The presence of pivot shift after anterior cruciate ligament (ACL) reconstruction is correlated with worse clinical outcomes. An orthopaedic navigation system is a useful tool for quantifying laxity in the ACL-deficient knee. PURPOSE: To investigate the relationship between preoperative knee laxity measured by a navigation system and postoperative pivot shift (PPS) after ACL reconstruction. STUDY DESIGN: Case-control study; Level of evidence, 3. METHODS: One hundred patients who underwent primary ACL reconstruction (62 hamstring tendon grafts, 38 patellar tendon grafts) were grouped according to the presence or absence of pivot shift at the 2-year follow-up, and the groups were compared retrospectively. Before surgery, knee laxity was assessed with a navigation system to quantify posterior tibial reduction (PTR) during pivot-shift tests and anterior tibial translation (ATT) during Lachman tests. PTR and ATT cutoff values were determined by receiver operator characteristic (ROC) analysis. RESULTS: Preoperative PTR and ATT were significantly larger for patients with PPS (PPS-positive group) than those without (PPS-negative group). In the ROC analysis, the PTR had an area under the curve of 0.871 (95% CI, 0.763-0.979; P < .0001) for predicting a PPS; this was larger than that obtained for the ATT, which had an area under the curve of 0.825 (95% CI, 0.705-0.946; P = .001). Because the ROC curve of the ATT had 2 peaks, the ATT alone was not a suitable predictor for PPS. Based on the ROC curve, the optimal PTR cutoff value was 7 mm, with 88.9% sensitivity and 71.4% specificity for PPS (adjusted odds ratio = 19.7; 95% CI, 2.1-187.9; P = .009). Setting the cutoff value as a combination of the PTR (≧7 mm) and ATT (≧12 mm) improved the specificity (88.9% sensitivity and 84.6% specificity; adjusted odds ratio = 149.8; 95% CI, 5.9-3822.7; P = .002) over that with the PTR alone. CONCLUSION: ACL injuries in knees with a large PTR had a higher risk of PPS. When reconstructing the ACL in a knee with a high degree of laxity, surgeons may need to adopt strategies to prevent PPS.

Characteristics of elongated and ruptured anterior cruciate ligament grafts: An analysis of 21 consecutive revision cases.

BACKGROUND/OBJECTIVE: Anterior cruciate ligament (ACL) reconstructions often fail without graft rupture. The purpose of this study was to compare the characteristics of patients with elongated and ruptured bone-patellar tendon-bone (BTB) grafts that required revision surgery. METHODS: Twenty one patients who required revisions of a BTB-reconstructed ACL between 2010 and 2015 were enrolled in this study. All patients were evaluated for bone tunnel position using computed tomography. Tunnel angle was calculated with radiographs. Stability under anaesthesia, and meniscus and cartilage condition were evaluated during the revision surgery. Age at primary surgery, time between primary and revision surgery, activity level, original tunnel position of the graft, and meniscus and cartilage condition were compared between elongated and ruptured grafts. RESULTS: Age at primary surgery was not significantly different between the two groups (p = 0.528). Time between primary and revision surgery as well as activity level were also not significantly different between the two groups (p = 0.010 and p = 0.307, respectively). Femoral bone tunnel position was more proximal (p = 0.003), and radiographic tunnel angle was not significantly different between the two groups (p = 0.029). The rupture group was significantly more unstable on the pivot shift (p < 0.003). Meniscus degeneration, meniscus tear, and cartilage damage were not significantly different between the two groups (p = 0.030, p = 0.311, and p = 0.505, respectively). CONCLUSION: The location of the original femoral tunnel was more proximal in patients with elongated grafts than in those with ruptured grafts. Different bone tunnel position from native ACL might lead to graft elongation.

Is Remnant Preservation Truly Beneficial to Anterior Cruciate Ligament Reconstruction Healing? Clinical and Magnetic Resonance Imaging Evaluations of Remnant-Preserved Reconstruction.

BACKGROUND: Remnant-preserved anterior cruciate ligament (ACL) reconstruction was introduced to improve clinical outcomes and biological healing. However, the effects of remnant preservation and the influence of the delay from injury until reconstruction on the outcomes of this technique are still uncertain. Purpose/Hypothesis: The purposes of this study were to evaluate whether remnant preservation improved the clinical outcomes and graft incorporation of ACL reconstruction and to examine the influence of the delay between ACL injury and reconstruction on the usefulness of remnant preservation. We hypothesized that remnant preservation improves clinical results and accelerates graft incorporation and that its effect is dependent on the delay between ACL injury and reconstruction. STUDY DESIGN: Cohort study; Level of evidence, 2. METHODS: A total of 151 consecutive patients who underwent double-bundle ACL reconstruction using a semitendinosus graft were enrolled in this study: 74 knees underwent ACL reconstruction without a remnant (or the remnant was <25% of the intra-articular portion of the graft; NR group), while 77 knees underwent ACL reconstruction with remnant preservation (RP group). These were divided into 4 subgroups based on the time from injury to surgery: phase 1 was <3 weeks (n = 24), phase 2 was 3 to less than 8 weeks (n = 70), phase 3 was 8 to 20 weeks (n = 32), and phase 4 was >20 weeks (n = 25). Clinical measurements, including KT-1000 arthrometer side-to-side anterior tibial translation measurements, were assessed at 3, 6, 12, and 24 months after reconstruction. Magnetic resonance imaging evaluations of graft maturation and graft-tunnel integration of the anteromedial and posterolateral bundles were assessed at 3, 6, and 12 months after reconstruction. RESULTS: There was no difference in side-to-side anterior tibial translation between the NR and RP groups. There was also no difference in graft maturation between the 2 groups. Furthermore, the time from ACL injury until reconstruction did not affect graft maturation, except in the case of very long delays before reconstruction (phase 4). Graft-tunnel integration was significantly increased in both groups in a time-dependent manner. However, there was no difference between the NR and RP groups. CONCLUSION: Remnant preservation did not improve knee stability at 2 years after ACL reconstruction. Furthermore, remnant preservation did not accelerate graft incorporation, especially during the acute and subacute injury phases.

Quantification of the pivot-shift test using a navigation system with non-invasive surface markers.

PURPOSE: The purpose of this study was to validate the quantitation of the pivot-shift phenomenon by using a navigation system with non-invasive surface markers. Measurements obtained using this system were compared with those obtained using commercial pin-fixed markers. METHODS: Seventy patients with anterior cruciate ligament (ACL) injuries were assessed under general anaesthesia. Knee kinematics during the pivot-shift test were recorded using an image-free navigation system with a commercial transmitter placed on the thigh and lower leg (surface markers) or those fixed to the femur and the tibia via metal pin fixators (pin-fixed markers). For quantitation of the pivot-shift phenomenon, posterior tibial reduction (PTR) was calculated using the two types of navigation system markers and were then compared. PTRs measured using the two types of markers were also compared with clinical grade of the pivot-shift test, as determined by an examiner. RESULTS: The pivot-shift phenomenon could be identified in all patients on the navigation screen. The PTR measured using surface markers moderately correlated with that measured using pin-fixed markers (ρ = 0.524, p < 0.001). There were also moderate correlations between clinical grades and the PTRs measured using either the surface markers (ρ = 0.522, p < 0.001) or the pin-fixed markers (ρ = 0.645, p < 0.001). CONCLUSIONS: The present study demonstrated that PTR, during the pivot-shift test, may be quantified in ACL-injured knees, using a navigation system with surface markers, and that the PTR measured with surface markers moderately correlated both with the PTR obtained using pin-fixed markers and with the clinical grade of the pivot-shift test. A customised method of fixing transmitters with reflective markers to patients' thighs and shins with Velcro straps is non-invasive and could assess and record the knee kinematics, especially the pivot-shift test, in ACL-injured and ACL-reconstructed knees before, during, and after surgery using a navigation system. LEVEL OF EVIDENCE: Case series, Level IV.

Quantitative analysis of dynamic patellar tracking in patients with lateral patellar instability using a simple video system.

BACKGROUND: As patellar dislocation occurs during activity, it is more important to assess the behavior of the patellofemoral joint under dynamic conditions. The aim of this study was to compare patellar tracking between knees with and without patellar dislocation in patients with an unstable patella and healthy controls using a simple video technique. METHODS: Twenty-three knees with patellar dislocation (dislocated group), 23 contralateral knees without dislocation (non-dislocated group), and 23 healthy knees (control group) were examined. Those with skin markers on anatomical landmarks were made to extend their knees actively, and skin markers were attached to the examiner's fingertips and the patella was followed by pinching. The knee during active knee extension was recorded with digital video cameras. The patella was tracked on imaging software, and the mediolateral patellar position (% patellar position: %PP) was calculated in reference to the knee width consecutively. RESULTS: %PP was significantly different between the dislocated and control groups, from 30° (mean±SD: 58.9±6.2%, 54.6±4.7%) to 5° (64.2±5.2%, 55.2±5.2%). It was also significantly different between the non-dislocated and control groups, from 25° (58.9±7.1%, 54.5±4.6%) to 5° (63.8±6.5%, 55.2±5.2%). No significant difference in %PP was found between the dislocated and non-dislocated groups. CONCLUSION: With the new video system, patellar tracking during active knee extension was successfully quantified. The tracking patterns were the same in knees with and without patellar dislocation, and the tracking in patients significantly differed from that in the controls at lower knee flexion angles. CLINICAL RELEVANCE: The development of a quantitative examination technique for dynamic patellar tracking, which is easy to use and repeatedly applicable in a clinical situation, could help to follow-up the time-dependent changes and analyze the treatment effect on an unstable patella.

Hyaluronic acid induces the release of growth factors from platelet-rich plasma.

BACKGROUND/OBJECTIVE: Platelet-rich plasma (PRP) and hyaluronic acid (HA) injection are both therapeutic options for osteoarthritis and chronic tendinopathy. Although several comparative studies on the two have been published, the effects of mixing PRP and HA are not fully understood. The purpose of this study is to investigate the influence of HA on platelets in PRP by measuring releasing growth factors. METHODS: PRP was produced from nine healthy adult volunteers (mean age, 32.8 ± 2.9 years; range, 29-37) with a commercial separation system. HA of weight-average molecular weight of 50-120 kDa was used. PRP group (PRP 1 mL + phosphate buffered saline 0.2 mL) and PRP + HA group (PRP 1 mL + HA 0.2 mL) were incubated at 37°C for 2 hours. The amounts of transforming growth factor ß1 (TGF-ß1) and platelet-derived growth factor (PDGF-AA) released from the PRP and PRP + HA samples were measured on Day 0, Day 3, and Day 5. In addition, the same growth factors on Day 5 were measured for PRP + high HA group (PRP 1 mL + HA 0.6 mL) with five donors. After collecting all of the samples on Day 5, the remaining gels were observed with Giemsa stain. Statistical analyses were performed using paired t tests to compare the PRP and HA groups at each time point, and a one-way analysis of variance (one-way ANOVA) with Tukey post hoc tests was used to compare the PRP, PRP + HA, and PRP + high HA groups. RESULTS: The TGF-ß1 concentrations in the PRP and PRP + HA were 24.3 ± 7.2 µg/mL and 22.4 ± 1.8 µg/mL (p = 0.689) on Day 0, 17.2 ± 13.9 µg/mL and 25.4 ± 7.1 µg/mL (p = 0.331) on Day 3, and 12.7 ± 10.5 µg/mL and 33.7 ± 8.3 µg/mL (p = 0.034) on Day 5. The TGF-ß1 concentrations on Day 5 were 24.1 ± 5.2 µg/mL (PRP group), 28.3 ± 2.4 µg/mL (PRP + HA), and 31.9 ± 4.8 µg/mL (PRP + high HA; one-way ANOVA: p = 0.003; post hoc PRP vs. PRP + HA: p = 0.016). The PDGF-AA concentrations in the PRP and PRP + HA groups were 2.30 ± 1.21 µg/mL and 2.32 ± 0.79 µg/mL (p = 0.931) on Day 0, 2.03 ± 0.53 µg/mL and 2.13 ± 0.73 µg/mL (p = 0.500) on Day 3, and 1.51 ± 0.40 µg/mL and 2.00 ± 0.52 µg/mL (p = 0.003) on Day 5. The PDGF-AA concentrations were 1.48 ± 0.46 µg/mL (PRP group), 1.94 ± 0.57 µg/mL (PRP + HA), and 2.69 ± 0.70 µg/mL (PRP + high HA; one-way ANOVA: p = 0.0002; PRP vs. PRP + high HA: p = 0.002; PRP + HA vs. PRP + high HA: p = 0.011) on Day 5. The PRP showed larger coagulated masses than the PRP + HA. The high concentration HA group had the smallest coagulated mass of all of the group. CONCLUSION: The levels of growth factors released by PRP on Day 5 were increased by the addition of HA. A mixture of PRP and HA may be a more effective therapy than PRP or HA alone for osteoarthritis and tendinopathy.

Periodic knee injections of collagen tripeptide delay cartilage degeneration in rabbit experimental osteoarthritis.

INTRODUCTION: Collagen peptides have been reported to possess various biological activities for various cell types. The purposes of this study were, first, to examine the therapeutic effects of collagen tripeptide (Ctp) in rabbit osteoarthritis and, second, to explore a synergetic effect with hyaluronan (HA). METHODS: Osteoarthritis was induced by anterior cruciate ligament transection of the right knee in 72 Japanese white rabbits and they were divided into four groups (control, Ctp, HA and Ctp/HA). Each material was injected weekly into the knee, and knee joint samples were collected 5, 10 and 15 weeks after surgery. Macroscopic and histomorphological analyses of cartilage were conducted. Expression of type II collagen and matrix metalloproteinase-13 was also analyzed immunohistochemically. A Tukey's honestly significant difference test was used to evaluate the statistical significance of difference in the macroscopic, histological and immnohistochemical results. RESULTS: All treatment groups exhibited slightly higher resistance to the progression of osteoarthritis than the control group macroscopically 15 weeks after surgery. Histologically, intra-articular injection of Ctp significantly reduced cartilage degradation 10 weeks after surgery, and Ctp/HA significantly reduced it 5 weeks after surgery in comparison with the control. Immunohistochemically, both Ctp-treated and Ctp/HA-treated groups had significantly increased type II collagen-positive chondrocytes at the fifth week after the surgery, although the numbers of matrix metalloproteinase-13-positive chondrocytes were not affected. CONCLUSION: Periodical injections of Ctp and Ctp/HA delayed progression of cartilage degeneration of early osteoarthritis induced by anterior cruciate ligament transection in rabbits. This effect appears to be exerted by promotion of type II collagen synthesis predominantly.

Tumor-necrosis factor-alpha induces retinoic acid-inducible gene-I in rheumatoid fibroblast-like synoviocytes.

Tumor-necrosis factor-alpha (TNF-alpha) is a potent proinflammtory cytokine and a key molecule in the pathogenesis of rheumatoid arthritis (RA). Retinoic acid-inducible gene-I (RIG-I) is a DExH box protein, which is known to play a role in the inflammatory and immune reactions. We previously reported about potential involvement of RIG-I in synovial inflammation in RA. In the present study, we demonstrated the expression of RIG-I in fibroblast-like synoviocytes stimulated with TNF-alpha. RNA interference against interferon (IFN)-beta abolished the TNF-alpha-induced RIG-I expression. In addition, knockdown of RIG-I partially inhibited the TNF-alpha-induced expression of CC chemokine ligand (CCL) 5, a chemokine with chemotactic activity toward lymphocytes and monocytes. These findings suggest that the TNF-alpha/IFN-beta/RIG-I/CCL5 pathway may be involved in the pathogenesis of synovial inflammation in RA.